May 27-28, 2019


Singapore City, Singapore

Conference Agenda

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Keynote Session:

Title: Alternative modality for detection of Entamoeba Species: A Comparative Analysis of Nested- Multiplex PCR and Recombinase Polymerase Amplification



Amoebiasis, a disease caused by Entamoeba histolytica, remains a serious public health issue worldwide. For decades, microscopy remains preferred modality for detection of the pathogenic species. Differentiation of Entamoeba histolytica and E. dispar still remains a challenge. The recent advent of molecular biology has helped in developing appropriate diagnostic technologies, of which isothermal amplification is considered to be robust and cost-effective compared to Polymerase chain reaction that requires expensive instruments and highly skilled personnel. The aim of the present study was to compare the diagnostic ability of nested-multiplex PCR and Recombinase polymerase amplification for differential detection of E. histolytica and E. dispar. The results of this study showed good agreement between the two tests. This suggests that RPA can be used as clinical and epidemiological tool for diagnosis of amoebiasis in resource-limited setups and in endemic regions